Haemoglobin adducts of aromatic amines: diamines and polyaromatic amines |
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Authors: | Gabriele Sabbioni Armin Beyerbach |
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Institution: | Walther-Straub-Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität München, Nußbaumstraße 26, D-80336 Munich, Germany |
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Abstract: | Aromatic amines and nitroarenes are important antioxidants and intermediates in the synthesis of dyes, pesticides and plastics. In the present paper we introduce methods for the synthesis of deuterated standards: 3-2H8]aminofluoranthene, 3,3′-dimethyl-2H4]benzidine, 2H4]benzidine, N′-acetyl-2H4]benzidine, 2,4-2H6]toluenediamine, 2,6-2H6]toluenediamine. These standards have been used for the quantification of haemoglobin adducts of diamines and polyaromatic amines. Haemoglobin was hydrolysed in 0.1 M sodium hydroxide and the hydrolysate extracted with dichloromethane. The extracts were derivatised with heptafluorobutyric anhydride and analysed by GC–MS with negative chemical ionisation. In one run up to 15 aromatic amines can be determined: 6-aminochrysene, 3-aminofluoranthene, 2-aminofluorene, 1-aminopyrene, benzidine, 3,3′-dichlorobenzidine, 3,3′-dimethoxybenzidine, 3,3′-dimethylbenzidine, 3,3′-methylenedianiline, 4,4′-methylenedianiline, N′-acetyl-benzidine, N′-acetyl-4,4′-methylenedianiline, 4,4′-methylene bis(2-chloroaniline), 2,4-toluenediamine and 2,6-toluenediamine. |
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Keywords: | Subject-index terms: Aromatic amines Haemoglobin adducts |
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