Determination of gestrinone in human serum by liquid chromatography–electrospray tandem mass spectrometry |
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Authors: | Qinggang Wang Zhuping Wu Yiming Wang Guoan Luo Erruo Wu Xuefeng Gao |
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Affiliation: | a Department of Chemistry, Tsinghua University, Beijing 100084, PR China;b National Research Institute for Family Planning, Beijing 100081, PR China |
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Abstract: | A rapid, sensitive and specific high-performance liquid chromatography–electrospray tandem mass spectrometric method has been developed for the determination of gestrinone (R 2323) in human serum using mifepristone (RU 486) as an internal standard. R 2323 was extracted from human serum by an ether extraction procedure. Multiple reaction monitoring was used to detect R 2323 and RU 486. The calibration curve was linear over the range of 3.5–177 ng/ml (r2≥0.99) with the limitation of detection of 0.8 ng/ml. The intra-day precision and accuracy, expressed as C.V. and RE, ranged from 2.3–13.7 to −4.8–3.0%. The inter-day precision and accuracy ranged from 5.5–14.8 to −6.7–3.1%. The mean recovery was 91.0% for R 2323, and 90.6% for the internal standard. The method was successfully applied to the pharmacokinetic study of R 2323. |
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Keywords: | Gestrinone |
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