Simple and reliable method of doxycycline determination in human plasma and biological tissues |
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Authors: | B. Axisa A. R. Naylor P. R. F. Bell M. M. Thompson |
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Affiliation: | Department of Surgery, Clinical Sciences Building, Leicester Royal Infirmary, Leicester LE2 7LX, UK |
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Abstract: | Over recent years there has been a resurgence in the use of doxycycline in clinical practice, which does not depend on its antibacterial properties. This paper describes a method of determination of doxycycline in human plasma and atheromatous tissue using high-performance liquid chromatography (HPLC), and a cheap commercially available extraction system. Doxycycline is extracted in the mobile phase and injected directly into the HPLC system, avoiding time consuming drying up steps. A limit of detection of 0.125 μg/ml of plasma, and a relative standard deviation of 3% was achieved, making the method very reliable and useful for assays within the usual therapeutic range. The method has also been applied to the extraction of a mixture of tetracyclines from plasma and atherma with equal efficacy, making it useful for assays of this class of drugs in veterinary practice and assays of food contaminants. |
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Keywords: | Doxycycline |
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