Quantification of phenytoin and its metabolites in equine plasma and urine using high-performance liquid chromatography |
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| Authors: | Fuyu Guan Cornelius E Uboh Lawrence R Soma Eric K Birks Donna Teleis Jeffrey A Rudy Anthony O Watson Deborah S Tsang |
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| Affiliation: | a University of Pennsylvania School of Veterinary Medicine Department of Clinical Studies, New Bolton Center Campus, Kennett Square, PA 19348, USA;b PA Equine Toxicology and Research Laboratory, Department of Chemistry, West Chester University, West Chester, PA 19382, USA |
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| Abstract: | A reliable and sensitive method for the extraction and quantification of phenytoin (5,5′-diphenylhydantoin), its major metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH) and minor metabolite, 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) in horse urine and plasma is described. The method involves the use of solid-phase extraction (SPE), liquid–liquid extraction (LLE), enzyme hydrolysis (EH) and high-performance liquid chromatography (HPLC). The minor metabolite, 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) was not present in a reliably quantifiable concentration in all samples. The new method described was successfully applied in the pharmacokinetic studies and elimination profile of phenytoin and p-HPPH following oral or intravenous administration in the horse. |
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| Keywords: | Phenytoin |
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