Simultaneous separation of atovaquone, proguanil and its metabolites on a mixed mode high-performance liquid chromatographic column |
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| Authors: | Y Bergqvist C Hopstadius |
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| Institution: | a Dalarna University College, S-781 88 Borlänge, Unit of Tropical Pharmacology, Division of Clinical Pharmacology, Karolinska Institute, Huddinge University Hospital, S-141 86 Huddinge, Sweden;b Department of Clinical Chemistry, Falun Central Hospital, S-791 82 Falun, Sweden |
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| Abstract: | An isocratic high-performance liquid chromatographic (HPLC) method for simultaneous separation of the components in the antimalarial combination drug Malarone® with UV detection is described. An HPLC system using a mixed mode column composed of 50% C18 phase and 50% strong cation-exchanger has been optimised for the simultaneous separation of atovaquone, proguanil and its two main metabolites. The mobile phase was optimised for factors such as pH, counter ion concentration and acetonitrile. Elimination of interferences from other antimalarial drugs was achieved by adding sodium perchlorate to the mobile phase. With a mobile phase of acetonitrile-phosphate buffer (60:40, v/v) pH 6.8, 50.7 mmol l−1 K+ and 10 mmol l−1 Na·ClO4, separation was achieved within a run time shorter than 17 min. |
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| Keywords: | Atovaquone Proguanil Malarone |
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