Two reproducible and sensitive liquid chromatographic methods to quantify atenolol and propranolol in human plasma and determination of their associated analytical error functions |
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Authors: | Antonio J. Braza, Pilar Modamio,Eduardo L. Mari o |
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Affiliation: | Clinical Pharmacy and Pharmacotherapy Unit, Department of Pharmacy and Pharmaceutical Technology, Health Division, Faculty of Pharmacy, University of Barcelona, Avda Joan XXIII s/n, 08028 -Barcelona, Spain |
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Abstract: | Two liquid chromatography (LC) methods with fluorimetric detection have been developed to measure atenolol and propranolol in human plasma. The same 5 μm Nucleosil RP-18 column, extraction procedure and mobile phase (containing acetonitrile, water, triethylamine and phosphoric acid, pH 3) were used. The linearity ranges were 25–800 ng/ml for atenolol and 3.13–100 ng/ml for propranolol. The coefficients of variation for validation assays were lower than 15% at the concentration assayed. The functions of the analytical error were linear: SD (ng/ml)=7.698+0.037C for atenolol and SD (ng/ml)=0.126+0.036C for propranolol. |
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Keywords: | Atenolol Propranolol β -Blockers |
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