光棘豆悬浮细胞原生质体培养再生小植株(简报)

迄今为止,药用植物原生质体培养再生植株的报道还不多。光棘豆(Oxytropis leptophylla)是多年生豆科植物,可饲用,是一种常用的野生中草药。全株入药有清热解毒功能,用于治疗痈疤肿毒。已有研究表明,光棘豆具有离体培养时愈伤组织增殖快、植株再生频率高的特点。进行原生质体培养再生植株的研究将有助于光棘豆的改良和驯化,为其开发利用提供基础。本文首次报道了光棘豆悬浮细胞原生质培养再生小植株的结果。

PLANTLET REGENERATION OF PROTOPLASTS DERIVED FROM CELL-SUSPENSION CULTURES OF OXYTROPIS LEPTOPHYLLA

This paper reports the plantlets regenerated from protoplasts of Oxytropis lep-tophyllay. Morphogenic suspension cultures were first established from the calli initiated from plumular axis of donor plants. Protoplasts were isolated enzymatically from these suspension cultures and were purified through filtration and centrifugation. They were cultured in thin-layer liquid medium of modified K 8 P, supplemented with fluidmedium CK 8.The minicalli formed through protoplast divisions were transferred into liquid medium S 12 for proliferation. When the morphogenic calli were formed, they were placed onto differentiation medium (NB 2,4-D 0.01mg/L, KT 4.0mg/L) for the induction of shoots. After the roots were induced on the medium (1/2 MS IBA 0.4mg/L, Suc. 2% ). the intact plant-lets were obtained.