Differential staining and chromatin packing of the mitotic chromosomes of the newt Triturus cristatus |
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Authors: | Edwina Rudak H. G. Callan |
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Affiliation: | (1) Department of Zoology, The University, KY16 9TS St. Andrews, Scotland |
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Abstract: | Mitotic metaphase chromosomes of cold-treated Triturus cristatus show a characteristic pattern of constrictions, most of which lie close, though not immediately adjacent, to the centromeres. The chromatin in these cold-induced constrictions stains intensely with Giemsa. Cold-treated spermatogonia show spiral structure throughout the metaphase chromatids; the packing of chromatin fibrils is much tighter in the constricted regions than elsewhere, and the gyres in the constricted regions are narrower and of shorter pitch. |
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