Changes in the distribution of mitochondria in mouse embryos blocked at the two-cell stage |
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Authors: | N. A. Bogolyubova |
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Affiliation: | (1) Institute of Cytology, Russian Academy of Sciences, Tikhoretskii pr. 4, 194064 St. Petersburg, Russia |
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Abstract: | Changes in the distribution of mitochondria in the two-cell mouse embryos preceding the developmental arrest in vitro, caused by a genetically determined two-cell block in vitro or genisteine treatment, were examined vitally using the mitochondrial-specific probe rhodamine 123 and conventional fluorescence microscopy. In the former case, serious disturbances in the localization of mitochondria appeared already from the middle of two-cell stage, long before the time corresponding to the 2nd cleavage division. Comparison of the behavior of mitochondria in the embryos successfully developing between the one- and two-cell stages and that in the embryos that ceased to cleave suggests that the developmental arrest was accompanied by aggregation of the mitochondria into clusters. There are many such clusters unlike in the cytoplasm of normally developing embryos. Intracellular localization of clusters observed in the genisteine-treated embryos differed radically from that observed in the embryos blocked in vitro at the two-cell stage.Translated from Ontogenez, Vol. 36, No. 1, 2005, pp. 51–60.Original Russian Text Copyright © 2005 by Bogolyubova. |
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Keywords: | mouse embryos preimplantation development two-cell stage two-cell block in vitro intracellular localization of mitochondria rhodamine 123 genisteine |
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