Molecular mapping in oil radish (Raphanus sativus L.) and QTL analysis of resistance against beet cyst nematode (Heterodera schachtii) |
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Authors: | Holger Budahn Herbert Peterka Magdi Ali Ahmed Mousa Yunhua Ding Shaosong Zhang Jinbin Li |
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Institution: | 1. Julius Kühn Institute, Institute for Breeding Research on Horticultural and Fruit Crops, Erwin-Baur-Stra?e 27, 06484, Quedlinburg, Germany 2. Horticulture Department, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt 3. Beijing Vegetable Research Centre, Academy of Agriculture and Forestry Sciences, 100097, Beijing, China 4. Yunnan Academy of Agricultural Sciences, Biotechnology Research Institute, 650223, Kunming, China
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Abstract: | The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil
radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration
to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition
of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated
markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted
of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome
d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed
as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish
linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F2 mapping population derived from a cross between varieties ‘Pegletta’ (nematode resistant) x ‘Siletta Nova’ (susceptible).
A dominant major QTL Hs1
Rph
explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments
to incorporate segments carrying desired genes as Hs1
Rph
from radish into rapeseed by means of chromosome addition lines. |
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