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Oxidative changes of lipids monitored by MALDI MS |
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| Authors: | Fuchs Beate Bresler Kristin Schiller Jürgen |
| Institution: | University of Leipzig, Faculty of Medicine, Institute of Medical Physics and Biophysics, Härtelstr. 16/18, D-04107 Leipzig, Germany |
| Abstract: | Oxidation processes of lipids are of paramount interest from many viewpoints. For instance, oxidation processes are highly important under in vivo conditions because molecules with regulatory functions are generated by oxidation of lipids or free fatty acids. Additionally, many inflammatory diseases are accompanied by lipid oxidation and, therefore, oxidation products are also useful disease (bio)markers. Thus, there is also considerable interest in methods of (oxidized) lipid analysis.Nowadays, soft ionization mass spectrometric (MS) methods are regularly used to study oxidative lipid modifications due to their high sensitivities and the extreme mass resolution. Although electrospray ionization (ESI) MS is so far most popular, applications of matrix-assisted laser desorption and ionization (MALDI) MS are increasing. This review aims to summarize the so far available data on MALDI analyses of oxidized lipids. In addition to model systems, special attention will be paid to the monitoring of oxidized lipids under in vivo conditions, particularly the oxidation of (human) lipoproteins. It is not the aim of this review to praise MALDI as the “best” method but to provide a critical survey of the advantages and drawbacks of this method. |
| Keywords: | Abbreviations: 9-AA 9-Aminoacridine amu Atomar mass unit AP Atmospheric pressure ATT 6-Aza-2-Thiothymine BHP Tert -butyl-hydroperoxide CL Cardiolipin ClCCA 4-Chloro-α-cyanocinnamic acid CHCA 4-Hydroxy-α-cyanocinnamic acid DHB 2 5-Dihydroxybenzoic acid DNA Deoxyribonucleic acid EI Electron ionization ESI Electrospray ionization ESR Electron spin resonance FT Fourier transform GC Gas chromatography HNE 4-Hydroxy-2-nonenal HPLC High performance liquid chromatography HSA Human serum albumine IgG Immunoglobulin G IR Infrared spectroscopy LC Liquid chromatography LD Laser desorption LOX Lipoxygenase LP Lipoprotein LPC Lysophosphatidylcholine LPL Lysophospholipid MALDI Matrix-assisted laser desorption and ionization MPO Myeloperoxidase MS Mass spectrometry m/z Mass over charge NADPH Nicotinamide adenine dinucleotide phosphate NMR Nuclear magnetic resonance PA Phosphatidic acid PC Phosphatidylcholine PE Phosphatidylethanolamine PG Phosphatidylglycerol PI Phosphatidylinositol pK Logarithm of acid dissociation constant PL Phospholipid ppm Parts per million PS Phosphatidylserine PSD Post source decay RNS Reactive nitrogen species ROS Reactive oxygen species SOD Superoxide dismutase SPME solid phase micro extraction TAG Triacylglycerol TBARS Thiobarbituric acid reactive substances TFA Trifluoroacetic acid THAP 2 4 6-Trihydroxyacetophenone (HP)TLC (High performance) thin-layer chromatography TOF Time-of-flight UV Ultraviolet |
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