Using fluorescence to locate intercalants within the lipid bilayer of liposomes, bioliposomes and erythrocyte ghosts |
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| Authors: | Afri Michal Naqqash Miriam E Frimer Aryeh A |
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| Affiliation: | The Ethel and David Resnick Chair in Active Oxygen Chemistry, Department of Chemistry, Bar Ilan University, Ramat Gan 52900 Israel |
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| Abstract: | In previous work, we have shown the utility of the “NMR technique” in locating intercalants within the lipid bilayer. We describe herein the development of a more sensitive and complementary “fluorescence technique” for this purpose and its application to liposomes, bioliposomes and erythrocyte ghosts. This technique is based on the observation in selected compounds of an excellent correlation between the emission wavelength (λem) and Dimroth–Reichardt ET(30) polarity parameter for the solvent in which the fluorescence emission spectrum was obtained. |
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| Keywords: | Abbreviations: ATR-FTIR, attenuated total reflectance-Fourier transform infrared DMPC, dimyristoylphosphatidylcholine EPR, electron paramagnetic resonance HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid NMR, nuclear magnetic resonance PBS, phosphate buffer solution RBC, red blood cell |
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