The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix |
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Authors: | E C Hurt B Pesold-Hurt G Schatz |
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Institution: | Biocenter, University of Basel, CH-4056 Basel, Switzerland |
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Abstract: | The cleavable prepiece of the precursor to yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) was attached to the amino-terminus of mouse dihydrofolate reductase (a cytosolic protein) by gene fusion. The resulting fusion protein was imported into the matrix of isolated, energized yeast mitochondria and cleaved to a polypeptide whose size was similar to that of authentic dihydrofolate reductase. |
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Keywords: | Mitochondrial protein import Yeast mitochondria Gene fusion Dihydrofolate reductase Precursor protein |
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