Solid phase peptide synthesis on hydrophilic supports. Part II--Studies using Perloza beaded cellulose. |
| |
Authors: | D R Englebretsen D R Harding |
| |
Affiliation: | Separation Science Unit, Massey University, Palmerston North, New Zealand. |
| |
Abstract: | Beaded cellulose (Perloza) was modified with acrylonitrile followed by reduction with diborane to give a functionalised support containing aminopropyl groups. This spacer arm was then further extended with a glycolamido or an Fmoc-amino acid-4-oxymethylphenoxyacetyl moiety. A number of peptides, including the Merrifield test peptide leucyl-alanyl-glycyl-valine, leucine-enkephalin, Acyl Carrier Protein (65-74), angiotensin I and II, ACTH(4-11) and LHRH were synthesised on the aminopropyl beaded cellulose support using modified t-butyloxycarbonyl (Boc) or fluorenylmethoxycarbonyl (Fmoc) synthesis protocols. The peptides were cleaved from the support and further purified. |
| |
Keywords: | |
|
|