Dealing with different methods for Kluyveromyces lactis β-galactosidase purification |
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| Authors: | M Becerra E Cerdán M I González Siso |
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| Institution: | 1. Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071, A Coru?a, Spain
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| Abstract: | Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining
of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native
enzyme. |
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