Low calcium environment effects osteoprotegerin ligand/osteoclast differentiation factor |
| |
Authors: | Takeyama S Yoshimura Y Shirai Y Deyama Y Hasegawa T Yawaka Y Kikuiri T Matsumoto A Fukuda H |
| |
Institution: | Oral Medicine and Diagnosis, Dental Pharmacology, Pediatric Dentistry, Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Kita13 Nishi 7, Kika-ku, Hokkaido, Sapporo, 060-8586, Japan. take@den.hokudai.ac.jp |
| |
Abstract: | In coculture with osteoblastic cell line MC3T3-E1 (E1) and mouse bone marrow cells, we reported that numbers of osteoclasts rose significantly on exposure to a low-calcium environment. Here we examined how osteoblasts influence osteoclastogenesis under a low-calcium environment. Comparing low extracellular calcium with a regular calcium environment, osteoprotegerin ligand (OPGL)/osteoclast differentiation factor (ODF) mRNA expression show more increase in the culture of low-calcium environment than in that of a regular calcium environment. Calcium-sensing receptor (CaSR), which was supposed as one of the mechanisms of recognizing extracellular calcium, existedon the surface of E1 cells. When E1 cells stimulated with agonists of CaSR, gadolinium, and neomycin, OPGL/ODF mRNA expression decreased. Moreover, these agonists reduced osteoclast formation in coculture. Taken together, it is possible that osteoblasts may recognize extracellular calcium via CaSR and regulate osteoclastogenesis. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|