Simple estimate of ureides in soybean tissue |
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| Authors: | T.G. Patterson R. Glenister T.A. LaRue |
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| Affiliation: | Boyce Thompson Institute, Tower Road, Ithaca, New York 14853 USA |
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| Abstract: | Extracts of soybean tissue are treated with an acidic cation-exchange resin to remove amino acids. The sample is treated with hypochlorite, pH 4.0, to convert the amide from allantoic acid or allantoin to products that react with alkaline phenol to form indophenol. The procedure is inexpensive, sensitive, and rapid. The method may be adapted for automated analysis with the Technicon autoanalyzer. |
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