Heterogeneity of urinary oligosaccharides from mannosidosis: Mass spectrometric analysis of permethylated Man9, Man8, and Man7 derivatives |
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Authors: | Heinz Egge Jean Claude Michalski Gerard Strecker |
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Affiliation: | 1. Institut für Physiologische Chemie der Universität Bonn, Nussallee 11, 5300 Bonn, German Federal Republic;2. Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille, Villeneuve D''Ascq, France |
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Abstract: | Three oligosaccharides isolated from the urine of a patient suffering from mannosidosis, a Man9GlcNAc, a Man8GlcNAc, and a Man7GlcNAc, were analyzed by electron impact mass spectrometry at 20 eV after reduction with NaB2H4 and permethylation. Molecular ions were observed at 2144, 1940, and 1736, respectively. In the high-mass range very intense ions were found at M-45. The mass spectrum of the homogenous decasaccharide Man9GlcNAc1D contains ions that could be attributed to specific parts of each of the three antennae of the molecule. Thus characteristic key ions were recognized. With the aid of these key ions the spectra of the nona- and octasaccharide mixtures could be evaluated in a qualitative and a semiquantitative way. In the nonasaccharide all three possible isomers that can be produced by cleavage of one of the three terminal α-mannoses are present, although in differing amounts. However, only five of the six possible isomers of the octasaccharide could be detected. |
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