Distribution of acetylated forms of nucleosomal histones in fractionated chromatin |
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| Authors: | Elena I. Georgieva Iliya G. Pashev Roumen G. Tsanev |
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| Affiliation: | Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria |
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| Abstract: | Hyperacetylated chromatin was isolated from Ehrlich ascites tumor cells grown in n-butyrate containing medium and fractionated by mild digestion with deoxyribonuclease II and precipitation with MgCl2. The most highly acetylated forms of histones H4 and H3 as well as the acetylated subspecies of H2B were found almost entirely associated with the putatively active Mg2+-soluble chromatin fraction. The Mg2+-insoluble fraction contained mainly mono- and diacetylated molecules of H4 and H3. |
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| Keywords: | To whom correspondence should be addressed. |
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