Effect of CuSO4 and Cu(II)(Gly)2 on some indirect assays for superoxide dismutase activity |
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Authors: | Elevter M Russanov Sofia G Ljutakova Stefan I Leutchev |
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Institution: | Institute of Physiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria |
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Abstract: | The effect of CuSO4 and Cu(II)(Gly)2 has been compared with that of superoxide dismutase on the ferricytochrome c reduction and on the nitroblue tetrazolium reduction by an enzymic or chemical flux of superoxide anion radicals as well as on o-dianisidine photooxidation. Both CuSO4 and Cu(II)(Gly)2 have been found to inhibit ferricytochrome c reduction as well as the aerobic and anaerobic nitroblue tetrazolium reduction with approximately equal efficiency. Unlike superoxide dismutase they proved capable of inhibiting o-dianisdine photooxidation. The effect of copper either as CuSO4 or as Cu(II)(Cly)2 has been established as being due to its interference with the indirect assays for superoxide dismutase activity used. The reasons for this interference have been examined and it is concluded that copper can react with a component of the indirect assay system and depending on the method used it either mimics SOD or acts contrary to the enzyme. |
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Keywords: | Author to whom all correspondence should be addressed: Institute of Physiology Bulgarian Academy of Sciences Acad G Bonchev St bl 1 1113 Sofia Bulgaria |
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