Characterization of microsatellite markers in peach [Prunus persica (L.) Batsch] |
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| Authors: | B. Sosinski M. Gannavarapu L. D. Hager L. E. Beck G.J. King C. D. Ryder S. Rajapakse W. V. Baird R. E. Ballard A. G. Abbott |
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| Affiliation: | (1) Department of Biological Sciences, Clemson University, Clemson SC 29634, USA e-mail: aalbert@clemson.edu Fax: 864-656-0435 Present address: B. Sosinski, Department of Plant Pathology, North Carolina State University, Raleigh NC 27695, USA, US;(2) Department of Horticulture, Clemson University, Clemson SC 29634, USA, US;(3) Department of Genetics, University of Georgia, Athens GA 30602, USA, GE;(4) Department of Plant Genetics and Biotechnology, Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, UK, GB |
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| Abstract: | Microsatellites have emerged as an important system of molecular markers. We evaluated the potential of microsatellites for use in genetic studies of peach [Prunus persica (L.) Batsch]. Microsatellite loci in peach were identified by screening a pUC8 genomic library, a λZAPII leaf cDNA library, as well as through database searches. Primer sequences for the microsatellite loci were tested from the related Rosaceae species apple (Malus×domestica) and sour cherry (Prunus cerasus L.). The genomic library was screened for CT, CA and AGG repeats, while the cDNA library was screened for (CT)n- and (CA)n-containing clones. Estimates of microsatellite frequencies were determined from the genomic library screening, and indicate that CT repeats occur every 100 kb, CA repeats every 420 kb, and AGG repeats every 700 kb in the peach genome. Microsatellite- containing clones were sequenced, and specific PCR primers were designed to amplify the microsatellite- containing regions from genomic DNA. The level of microsatellite polymorphism was evaluated among 28 scion peach cultivars which displayed one to four alleles per primer pair. Five microsatellites were found to segregate in intraspecific peach-mapping crosses. In addition, these microsatellite markers were tested for their utility in cross-species amplification for use in comparative mapping both within the Rosaceae, and with the un- related species Arabidopsis thaliana L. Received: 18 June 1999 / Accepted: 6 December 1999 |
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| Keywords: | Rosaceae Peach Prunus Microsatellite Simple sequence repeats |
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