| 预处理方式对微紫青霉菌吸附Cu2+的影响及其吸附机理 |
| |
| 引用本文： | 黄晓婷,孙长斌,陈小玲,秦会娟,胡美,袁媛,李有志. 预处理方式对微紫青霉菌吸附Cu2+的影响及其吸附机理[J]. 生物工程学报, 2009, 25(1): 0076-0083 |
| |
| 作者姓名： | 黄晓婷 孙长斌 陈小玲 秦会娟 胡美 袁媛 李有志 |
| |
| 作者单位： | 1. 广西大学生命科学与技术学院,南宁,530005;微生物及植物遗传工程教育部重点实验室,南宁,530005;广西亚热带生物资源保护利用重点实验室,南宁,530005
2. 广西大学生命科学与技术学院,南宁,530005 |
| |
| 基金项目： | 广西科技攻关项目(Nos. 0895003-8和0443001-20), 广西亚热带生物资源保护利用重点实验室主任基金(No. 07-04) 和微生物与植物遗传工程教育部重点实验室开放基金(No. J0701)资助。 |
| |
| 摘 要： | 为有效提高青霉菌(Penicillium)对含Cu2+的水溶液中的Cu2+的吸附能力并了解其吸附机制, 研究了8种不同预处理方式对微紫青霉菌(Penicillium janthinellum)菌株GXCR的Cu2+吸附的影响。结果表明, 匀浆化、匀浆+碱化(NaOH) (简称匀浆碱化)、高温(80oC)、匀浆+盐化(NaCl)、匀浆+洗涤剂和匀浆+极化(二甲基亚砜)可显著提高菌体的吸附率, 但是匀浆化+酸化处理会导致菌体的Cu2+吸附能力显著下降。与早期其他研究相比, 本研究发现匀浆化碱化(NaOH)相结合的方式能够显著提高菌体的吸附能力。其中匀浆碱化(NaOH, 0.5 mol/L)处理后菌体的Cu2+的吸附率增加了47.95%; 匀浆碱化菌体吸附符合典型的Langmuir方程, 表明该菌对吸附Cu2+的吸附可能是以表面吸附为主。吸附-解吸循环4次后, 碱化菌体的Cu2+的吸附率仍可达到70.82%。红外光谱分析表明碱化处理主要影响菌体表面分子的-OH、C=O和COOH基团中的C=O, 其中与Cu2+结合的主要基团是-OH。GXCR的对Cu2+的吸附可能是主要基于Cu2+与菌体-OH基团结合的化学吸附为主。
|
| 关 键 词： | 真菌 预处理 生物吸附 解析 吸附机理 |
| 收稿时间： | 2008-05-16 |
Effects of pre-treatment on Cu2+ absorption of Penicillium janthinellum strain GXCR |
| |
| Xiaoting Huang,Changbin Sun,Xiaoling Chen,Huijuan Qin,Mei Hu,Yuan Yuan and Youzhi Li. Effects of pre-treatment on Cu2+ absorption of Penicillium janthinellum strain GXCR[J]. Chinese journal of biotechnology, 2009, 25(1): 0076-0083 |
| |
| Authors: | Xiaoting Huang Changbin Sun Xiaoling Chen Huijuan Qin Mei Hu Yuan Yuan Youzhi Li |
| |
| Affiliation: | College of Life Science and Technology, Guangxi University, Nanning 530005, China; Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Nanning 530005, China; Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization at Guangxi University, Nanning 530005, China;College of Life Science and Technology, Guangxi University, Nanning 530005, China; Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Nanning 530005, China; Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization at Guangxi University, Nanning 530005, China;College of Life Science and Technology, Guangxi University, Nanning 530005, China; Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Nanning 530005, China; Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization at Guangxi University, Nanning 530005, China;College of Life Science and Technology, Guangxi University, Nanning 530005, China; Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Nanning 530005, China; Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization at Guangxi University, Nanning 530005, China;College of Life Science and Technology, Guangxi University, Nanning 530005, China;College of Life Science and Technology, Guangxi University, Nanning 530005, China;College of Life Science and Technology, Guangxi University, Nanning 530005, China; Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Nanning 530005, China; Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization at Guangxi University, Nanning 530005, China |
| |
| Abstract: | In order to effectively increase capacity of Cu2+ absorption by Penicillium from Cu2+-containing aqueous solution and to study the mechanisms of absorption,effects of eight pre-treatment methods on Cu2+ absorption of Penicillium janthinellum strain GXCR were compared.The results showed that the efficiency of Cu2+ absorption obviously increased through pre-treatment by homogenization,homogenization-basification(NaOH),oven dry(80oC),homogenization-salinification(NaCl),homogenization-detergent and homogenizati... |
| |
| Keywords: | Cu2 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
|
点击此处可从《生物工程学报》下载全文 |
|
