A straight correlation between mutagenic activity and beta-galactosidase activity induced by monofunctional alkylating agents. |
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Authors: | M Ohtsuka K Maekawa |
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Affiliation: | Hita Research Laboratories, Chemicals Inspection and Testing Institute, Oita, Japan. |
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Abstract: | Eight monofunctional alkylating agents were examined for their ability to induce mutation in Salmonella typhimurium. The assay was carried out in S. typhimurium TA100 with the preincubation method. The SN1-type agents were more mutagenic than the SN2-type ones; besides, methylating agents exerted more mutagenic activity than ethylating ones. Those responses in the reversion assay were quite similar to the results obtained previously with the beta-galactosidase assay in Escherichia coli CSH26/pMCP1000 (alkA'-lacZ') as to the induction of the adaptive response. A good correlation was found between mutagenic potency in the reverse mutation assay and inducing potency in the beta-galactosidase assay. |
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