LncRNA RP1调控周期蛋白质促进结肠癌细胞增殖

长链非编码RNAs（long non-coding RNAs, lncRNAs）是一类无蛋白质编码功能，长度大于 200 nt的RNAs。qRT-PCR实验证实，lncRNA RP1-506.5（命名为RP1）在人结肠癌细胞株中的表达量明显高于人正常结肠上皮细胞（P<0.01）。RP1在结肠癌组织中的表达量为癌旁组织中表达量的8.5倍。在HCT116中，上调RP1的表达，同时在HCT8中沉默RP1的表达，探讨RP1对结肠癌细胞生物学特性的影响。MTS实验、活细胞工作站增殖实验，结合平板克隆实验发现，过表达RP1能明显促进结肠癌细胞HCT116的增殖能力。而在HCT8细胞中沉默RP1表达后，该细胞的增殖能力明显减弱。流式细胞周期实验结果表明，RP1能促进细胞周期快速通过G1/S检测点，并能加速S期进程。荧光定量PCR、Western印迹实验发现，在HCT116中细胞中，上调RP1的表达后，P21的表达水平下调，细胞周期蛋白D1（cyclinD1）、依赖细胞周期蛋白激酶6(CDK6)表达水平上调；当沉默LncRNA RP1的表达后，能上调P21的表达水平，下调cyclinD1、CDK6的表达水平。这些结果表明，LncRNA RP1可通过调控周期相关蛋白质的表达促进结肠癌细胞增殖。

LncRNA RP1 Promotes the Proliferation of Colon Cancer Cells by Regulating Cell Cycle Proteins

Long-chain non-coding RNAs (LncRNAs) is a class of non-protein coding RNA with length larger than 200 nt. The expression levels of LncRNA RP1-506.5 (named RP1) in human colon cancer cell lines were significantly higher than that in human normal colon epithelial cells by qRT-PCR assay. The expression levels of RP1 in colon cancer tissues were 8.5 folds of adjacent normal tissues. The expression of RP1 was overexpressed in HCT116 and knocked down in HCT8 to investigate the effect of RP1 on the proliferation of colon cancer cells. MTS assay, proliferation experiment in Live Cells Workstation combined with colony formation assay suggested that overexpression of RP1 could promote the proliferation of colon cancer cells, while knock down of RP1 remarkably inhibited cell proliferation. Flow cytometry showed that RP1 could promote cell cycle passing through the G1/S check point quickly and accelerate S phase. Furthermore, qRT-PCR and Western blot analysis demonstrated that RP1 decreased P21 and increased cyclin D1 and cyclin-dependent kinase 6 (CDK6) at both mRNA and protein levels. These results suggested that lncRNA RP1 could promote the proliferation of colon cancer cells by regulating the expression of cyclin related proteins.