A host-dependent hybrid plasmid suitable as a suicidal carrier for transposable elements |
| |
| Authors: | Mamoru Sato Brian J Staskawicz Nickolas J Panopoulos Sasha Peters Mary Honma |
| |
| Institution: | Department of Plant Pathology, University of California, Berkeley, California 94720 USA |
| |
| Abstract: | Plasmid pAS8Tcs rep-1::Tn7 (abbreviated pAS8Rep-1), a derivative of the RP4-ColE1 hybrid plasmid pAS8 displaying ColE1-dependent replication/maintenance, was found capable of the introduction of transposon Tn7 into the genome of phytopathogenic Pseudomonas. The plasmid is potentially useful as a general purpose suicidal Tn carrier for bacteria that do not support stable replication/maintenance of ColE1 but are within the conjugational host range of RP4. |
| |
| Keywords: | To whom reprint requests should be addressed |
| 本文献已被 ScienceDirect 等数据库收录！ |
