Degradation of cellulases in cultures of Cellulomonas fimi |
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Authors: | L.E. Sandercock A. Meinke N.R. Gilkes D.G. Kilburn R.A.J. Warren |
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Affiliation: | Department of Microbiology and Immunology, University of British Columbia, 00-6174 University Blvd., Vancouver B.C. V6T 1Z3, Canada |
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Abstract: | Abstract Endoglucanases CenA, CenB and CenD, cellobiohydrolases CbhA and CbhB, and the mixed function xylanase-exoglucanase Cex are degraded proteolytically in the supernatants of cultures of Cellulomonas fimi growing with cellulose. All of these polypeptides are modular. The initial sites of proteolysis are within or adjacent to the linkers connecting the modules, leading to the appearance of discrete fragments of the enzymes which retain the functions of the component modules. |
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Keywords: | Cellulomonas Cellulase Degradation Proteolysis |
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