Subcellular Localization of "Peripheral-Type" Binding Sites for Benzodiazepines in Rat Brain |
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Authors: | Anthony S. Basile Phil Skolnick |
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Affiliation: | Laboratory of Bioorganic Chemistry, National Institute of Arthritis, Digestive, Diabetes and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, U.S.A. |
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Abstract: | The binding of [3H]Ro 5-4864, a specific ligand for "peripheral-type" benzodiazepine binding sites and [3H]Ro 15-1788, a specific ligand for the central benzodiazepine receptors, was determined in subcellular fractions of rat brain. As previously reported, the highest levels of "peripheral-type" benzodiazepine binding sites and benzodiazepine receptors were found in the crude P1 and P2 fractions, respectively. Purification of these crude fractions revealed that high levels of both [3H]Ro 5-4864 and [3H]Ro 15-1788 binding were present in the mitochondrial and synaptosomal fractions. In contrast, the purified nuclei and myelin contained low levels of both [3H]Ro 5-4864 and [3H]Ro 15-1788 binding. |
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Keywords: | Ro 5–4864 Ro 15–1788 Benzodiazepine receptors Nuclei " Peripheral-type" binding site |
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