| TN-C在小细胞肺癌中的表达及STAT3对TN-C表达的影响 |
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| 引用本文： | 陈永锋,王海晶,刘丁媛,周向东. TN-C在小细胞肺癌中的表达及STAT3对TN-C表达的影响[J]. 生物磁学, 2014, 0(18): 3429-3432 |
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| 作者姓名： | 陈永锋 王海晶 刘丁媛 周向东 |
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| 作者单位： | 第三军医大学西南医院呼吸科,重庆400038 |
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| 摘 要： | 目的：探讨小细胞肺癌（SCLC）组织和小细胞肺癌细胞（H446）中肌糖蛋白-C（TN-C）的表达及STAT3对TN-C表达的影响。方法：应用免疫组化法检测58例小细胞肺癌和17例癌旁正常组织中TN-C的表达水平,应用RT-PCR和Western blotting法检测STAT-siRNA和STAT3过表达的H446细胞中TN-C的表达水平。结果：（1）小细胞肺癌组织中TN-C的表达水平显著高于癌旁正常组织（P〈0.05）;（2）在H446细胞中,TN-C和STAT3均呈现高表达;（3）STAT3-siRNA处理的H446细胞中STAT3和TN-C的表达均显著降低（P〈0.05）,而STAT3过表达的H446细胞中STAT3和TN-C的表达均显著上调（P〈0.05）。结论：TN-C在小细胞肺癌中的表达上调,可能受到STAT3的调控。
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| 关 键 词： | 小细胞肺癌 信号传导与转录激活因子3 肌糖蛋白-C |
Expression of TN-C in small cell lung cancer and the effect of STAT3 on TN-C Expression |
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| CHEN Yong-feng,WANG Hai-jing,LIU Ding-yuan,ZHOU Xiang-dong. Expression of TN-C in small cell lung cancer and the effect of STAT3 on TN-C Expression[J]. Biomagnetism, 2014, 0(18): 3429-3432 |
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| Authors: | CHEN Yong-feng WANG Hai-jing LIU Ding-yuan ZHOU Xiang-dong |
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| Affiliation: | (Department of respiration, Southwest hospital of the third military medical university, Chongqing, 400038, China) |
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| Abstract: | Objective： To explore the expression of TN-C in small cell lung cancer tissues and the influence of STAT3 on TN-C expression in H446 cells. Methods： The level of TN-C in 58 cases of SCLC tissues and 17 cases of adjacent normal lung tissue were determined by immunohistochemistry, and the TN-C expression were also detected by RT-PCR and western blotting in STAT3-siRNA or STAT3 overexpression H446 cells. Results： Significant up-regulation of TN-C in SCLC tissues was observed comparing with normal adjacent tissues. Both the TN-C and STAT3 expression were up-regulated in H446 cells. The STAT3 and TN-C expression were both decreased in STAT3-siRNA H446 cells but increased in STAT3 overexpressed H446 cells （P〈0.05）. Conclusion： Expression of TN-C was up-regulated in SCLC, which may be mediated by STAT3. |
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| Keywords: | Small cell lung cancer STAT3 TN-C |
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