MiR-30a-3p对人滋养肿瘤细胞系JEG-3细胞侵袭功能的影响

目的：MiRNAs对于胎盘的形成和正常妊娠的维持起着至关重要的作用,它在胎盘中的表达失衡的可能导致了妊娠相关疾病的发生,我们前期研究发现miR-30a-3p在子痫前期患者胎盘上特异性高表达,推测miR-30a-3p可能参与了子痫前期的发生发展过程,本课题通过观察miR-30a-3p对人滋养肿瘤细胞系JEG-3细胞侵袭能力的影响,深入探讨miR-30a-3p在子痫前期发病过程中的作用。方法：应用瞬时转染技术在人滋养肿瘤细胞系JEG-3细胞中分别转染miR-30a-3p mimics、mimics NC为miR-30a-3p过表达组和阴性对照组,空白转染组为空白对照组,利用荧光实时定量PCR技术检测各组细胞中miR-30a-3p的表达,Transwell实验检测各组细胞侵袭能力的差别。结果：荧光实时定量PCR结果显示miR-30a-3p过表达组与阴性对照组、空白对照组相比miR-30a-3p的表达量明显升高,差异具有统计学意义（P〈0.05）;Transwell实验结果显示miR-30a-3p过表达组细胞的侵袭能力与阴性对照组、空白对照组相比均有降低,差异具有统计学意义（P〈0.05）。阴性对照组与空白对照组的侵袭能力差异无统计学意义（P〉0.05）。结论：miR-30a-3p可以显著下调JEG-3细胞的侵袭力,miR-30a-3p有可能通过降低滋养细胞的浸润能力,导致滋养细胞对子宫肌层和螺旋动脉的浸润不足,造成＂胎盘浅着床＂,从而在子痫前期的发病过程中发挥了重要的作用,miR-30a-3p有望成为诊治子痫前期疾病的靶点。

Effect of MiR-30a-3p on Invasive Capacity of Choriocarcinoma Cell Line JEG-3

Objeeitve： MiRNAs plays an important role in the formation of the placenta and the maintenance of normal pregnancy, its imbalanced expression in placenta may lead to a pregnancy related to the occurrence of diseases, our previous study found that miR-30a-3p specificity high expression in the placenta of patients with preeclampsia, speculated that miR-30a-3p may participate in the development process of preeclampsia, our study observe the effect of miR-30a-3p on the invasive capactiy of choriocarcinoma cell line JEG-3 to identify the fimction ofmiR-30a-3p in the process ofpre-eclampsia. Methods： The technology of transient transfection was used to transfect with miR-30a-3p mimics and mimics NC in JEG-3 cells as miR-30a-3p overexpression goup and the negative control group, blank transfection group as the blank control group.The expression of miR-30a-3p was detected by Real time PCR. The invasive capacity of JEG-3 were checked by transwell assay.Results： Real time PCR results showed that the expression ofmiR-30a-3p displayed a marked increase after transfected with miR-30a-3p mimcs in JEG-3 cell （P〈0.05）. miR-30a-3p promoted the invasive capacity of JEG-3 cells more obvious in study group than that in conrol groups （P〈0.05）. Conclusions miR-30a-3p can significantly reduce the invasive capacity of the JEG-3, miR-30a-3p may lead to nourish cells of myometrium and invasion of the spiral arteries by reducing the sertoli cell infiltration ability, causing＂shallow placenta implantation＂, so it could be the diagnosis and therapeutic target in the treatment of preeclampsia.