Hormonal requirements for the induction of cytochrome P-450 in hepatocytes cultured in a serum-free medium |
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Authors: | J F Sinclair P R Sinclair H L Bonkowsky |
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Affiliation: | 1. VA Medical & Regional Office Center White River Junction, Vermont 05001 USA;2. Department of Biochemistry, Dartmouth Medical School Hanover, New Hampshire 03755 USA;3. Department of Medicine, Dartmouth Medical School Hanover, New Hampshire 03755 USA |
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Abstract: | Drug mediated induction of cytochrome P450 was studied in cultures of hepatocytes that had never been cultured in the presence of serum. Propylisopropylacetamide induced a five-fold increase in cytochrome P450, approximating induced levels, when triiodothyronine and/or dexamethasone were included in the culture medium. Insulin was apparently not required for this induction. Cytochrome P450, free of cytochrome oxidase, could be fully recovered from cell homogenates in a 8700g supernatant, by use of a buffer containing 0.2% Emulgen. |
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Keywords: | WE Williams E medium P450 Cytochrome P450 Dex Dexamethasone T3 3,3′,5-Triiodothyronine δ-ALAS δ-aminolevulinate synthase β-NF β-naphthoflavone PIA Propylisopropyl-acetamide |
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