铁过负荷对大鼠眼、泪腺中铁血黄素的沉积及转铁蛋白表达的影响

通过饲喂高铁饲料、腹腔注射或静脉注射多糖铁复合物的方法复制SD(Sprague Dawley)大鼠(Rattus norregicus)的铁过负荷模型。取动物的眼和泪腺组织染色显微观察发现,静脉注射多糖铁复合物大鼠眼巩膜、虹膜、睫状体、脉络膜间质细胞及泪腺小叶间结缔组织中巨噬细胞铁血黄素沉积明显;而腹腔注射多糖铁复合物或饲喂高铁饲料大鼠的眼和泪腺中铁血黄素沉积较少或未见沉积。不同方法造成的铁过负荷大鼠眼和泪腺组织中均未见转铁蛋白的表达。结果表明,铁血黄素在眼及泪腺的沉着很可能是眼晶状体混浊和眼底血管样条纹产生的主要原因。

Iron Deposition and Transferrin Expression in the Eye and Lacrimal Glands in Iron Overload Rats

In order to achieve hemosiderosis,the rats were fed with iron-supplemented diet or injected with iron polysaccharide complex intraperitoneally(i.p.) or intravenously(i.v.).Hemosiderin deposit was detected in the sclera,iris,ciliary body,and interstitial connective tissue of the choroid and macrophages lying in the interlobular connective tissue of lacrimal glands in all animals that received iron i.v.Also,little or no iron deposit was detected in the eye and lacrimal glands in animals that received iron i.p...