| 红细胞生成刺激素(EPO)的纯化 |
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| 引用本文： | 蔡辉国,马双,许静,汤晓培,王爱萍. 红细胞生成刺激素(EPO)的纯化[J]. 中国生物化学与分子生物学报, 1992, 8(3): 311-315 |
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| 作者姓名： | 蔡辉国 马双 许静 汤晓培 王爱萍 |
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| 作者单位： | 中国医学科学院血液学研究所，中国医学科学院血液学研究所，中国医学科学院血液学研究所，中国医学科学院血液学研究所，中国医学科学院血液学研究所 天津 300020，天津 300020，天津 300020，天津 300020，天津 300020 |
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| 摘 要： | 本文用中空纤维柱超滤浓缩尿,再经离子交换层析、聚焦层析、凝胶过滤和制备型聚丙烯酰胺凝胶电泳(PAGE)-层析等四步从15L再生障碍性贫血患者尿中获得约2mg EPO制品。比活性达10 300U/mg蛋白。该制品在分析型PAGE中呈一条区带。
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| 关 键 词： | 红细胞生成刺激素 蛋白质纯化 |
| 收稿时间： | 1992-06-20 |
Purification of Erythropoietin |
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| Cai,Hui-guo Ma,Shuang Xu,Jing Tang,Xiao-pei Wang,Ai-ping. Purification of Erythropoietin[J]. Chinese Journal of Biochemistry and Molecular Biology, 1992, 8(3): 311-315 |
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| Authors: | Cai Hui-guo Ma Shuang Xu Jing Tang Xiao-pei Wang Ai-ping |
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| Affiliation: | (Institute of Hematology,Chinese Academy of Medical Sciences,Tianjing 300020 |
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| Abstract: | This paper reported a purification method of erythropoietin (EPO) derived from human urine of patients with aplastic anemia.Urine was ultrafiltrated using a hollowfiber ultrafilter, and then followed by a four-step procedure, which included ion exchange chromatography, chromatofocusing, gel filtration, and preparative pol-yacrylamide gel electrophoresis (PAGE)-chromatography, A yield of about 2 mg EPO preparation with a potency of 10 300 units/mg protein from 15 liters of urine was obtained.The purified hormone has a single component in analytical PAGE. |
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| Keywords: | Erythropoietin Protein purification |
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