Simple and rapid screening method for microbial D-stereospecific peptidase and esterase |
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| Authors: | Seok-Joon Kwon Dae-Heoun Baek Seung-Goo Lee Moon-Hee Sung |
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| Affiliation: | (1) Microbial Conversion Research Unit, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, 305-600, Korea |
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| Abstract: | The use of a turbid plate containing Z-D-Ala-D-AlaOBzl was a convenient method for the screening of microorganisms with D-stereospecific peptidase activity as well as D-stereospecific esterase activity. This paper discusses the suitability of the developed method and its application to molecular cloning of D-stereospecifc peptidase and esterase genes. |
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| Keywords: | D-amino acids D-peptides esterases peptidases stereospecificity |
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