| 濒危植物元宝山冷杉的遗传多样性研究 |
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| 引用本文： | 王燕,唐绍清,李先琨. 濒危植物元宝山冷杉的遗传多样性研究[J]. 生物多样性, 2004, 12(2): 269-273 |
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| 作者姓名： | 王燕 唐绍清 李先琨 |
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| 作者单位： | 1. 广西师范大学生物系,桂林,541004
2. 广西壮族自治区,广西植物研究所,桂林,541006;中国科学院,广西植物研究所,桂林,541006 |
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| 基金项目： | 国家自然科学基金(39960018)，广西自然科学基金(桂科自9811013) |
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| 摘 要： | 元宝山冷杉(Abies yuanbaoshanensis)是仅分布于广西融水县元宝山的珍稀濒危物种。本研究采用AFLP分子标记分析了元宝山冷杉种群的遗传多样性。取元宝山冷杉种群43棵植株作为研究材料，4对引物组合用于AFLP扩增，共得到261条DNA扩增带。分析结果：元宝山冷杉种群的多态位点百分比率为50．96％，Nei's基因多样性为0．1510，Shannon多样性指数为0．1735。用NTSYS软件计算各个样品间的相似性系数，并用UPGMA法基于相似性系数进行了聚类分析。研究结果表明：元宝山冷杉种群的遗传多样性水平低，种群内个体间的相似性系数很大，无明显亚种群间的遗传分化。要保护或保存这个物种的遗传资源，建议应该选择种群内尽可能多的个体进行保护。
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| 关 键 词： | 濒危植物 元宝山冷杉 遗传多样性 AFLP 广西融水县元宝山 种群 |
| 文章编号： | 1005-0094(2004)02-0269-05 |
| 收稿时间： | 2003-06-12 |
| 修稿时间： | 2003-06-12 |
The genetic diversity of the endangered plant Abies yuanbaoshanensis |
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| WANG Yan,TANG Shao-Qing,LI Xian-Kun. The genetic diversity of the endangered plant Abies yuanbaoshanensis[J]. Biodiversity Science, 2004, 12(2): 269-273 |
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| Authors: | WANG Yan TANG Shao-Qing LI Xian-Kun |
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| Affiliation: | WANG Yan,TANG Shao-Qing,LI Xian-KunDepartment of Biology,Guangxi Normal University,Guilin 541004Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006 |
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| Abstract: | Abies yuanbaoshanensis (Pinaceae) is an endangered species endemic to Yuanbaoshan Mountain , Guangxi, China. Genetic diversity in 43 individuals of the speices was assessed using the amplified fragment length polymorphisms (AFLP) technique. Four primer combinations generated a total of 261 bands, of which 133 were polymorphic (50. 96%). The Nei's gene diversity of the population was 0. 1510, and Shannon's information index was 0. 1735. The results indicate that Abies yuanbaoshanensis has a low level of genetic diversity. This finding provides valuable information for formulating future conservation programs for the species. |
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| Keywords: | Abies yuanbaoshanensis AFLP genetic diversity conservation |
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