Bioreduction of nitroxides by Staphylococcus aureus. |
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Authors: | J S Goldberg E J Rauckman G M Rosen |
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Institution: | Department of Pharmacology Duke University Medical Center Durham, North Carolina 27710 USA |
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Abstract: | Two nitroxide radicals (TEMPO, I; OXAN, II) and a spin labeled penicillin (III) were reduced by . A short induction period preceded zero order reduction of these substrates leading to a Km of 8 × 10?4M, 6.67 × 10?5M and 5.7 × 10?4M and Vmax of 106, 26 and 11 μ mole/min mg bacteria for I, II and III, respectively. |
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