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95
Development of macroalgal (seaweed) taxonomic keys utilizing digital & media technology
Authors:T F Lee  P Kugrens
Abstract:Specimens of Codium fragile (Suringar) Hariot ssp. tomentosoides were collected from 9 sites in New England, and Long Island, New York at intervals throughout the years 1999‐2003. Segments were removed from the thalli and chopped into fine fragments, mostly individual utricles and medullary filaments. Fragments were incubated in enriched seawater in dim light at 15C, 12:12 LD. Within 2–3 days, in almost all cases (more than 300) motile cells formed in many of the utricles and filaments. These were 10–15 micrometer elongated biflagellate heterokont cells. They appeared to consume the chloroplasts, and within 24 hours were reduced to colorless cells, about 5 micrometers long. These cells are unable to grow in Codium chloroplast suspensions. They appear to be always associated with Codium thalli, despite attempts to clean the thalli, and were never seen in utricles or filaments of intact plants. Their ultrastructure is under investigation and will be reported on here.
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