Catalase T and Cu,Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae |
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| Authors: | Guaragnella Nicoletta Antonacci Lucia Giannattasio Sergio Marra Ersilia Passarella Salvatore |
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| Affiliation: | Dipartimento di Scienze per la Salute, Università del Molise, Via De Sanctis I, Campobasso, Italy. |
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| Abstract: | To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA-PCD), we compared Saccharomyces cerevisiae cells (C-Y) and cells individually over-expressing catalase T (CTT1-Y) and Cu,Zn-SOD (SOD1-Y) with respect to cell survival, hydrogen peroxide (H2O2) levels and enzyme activity as measured up to 200 min after AA treatment. AA-PCD does not occur in CTT1-Y, where H2O2 levels were lower than in C-Y and the over-expressed catalase activity decreased with time. In SOD1-Y, AA-PCD was exacerbated; high H2O2 levels were found, SOD activity increased early, remaining constant en route to AA-PCD, but catalase activity was strongly reduced. |
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| Keywords: | AA, acetic acid AA-PCD, acetic acid-induced PCD CHX, cycloheximide CTT1, catalase T DCF, dichorofluorescein H2-DCF-DA, 2,7-dichlorodihydrofluorescein diacetate H2O2, hydrogen peroxide PCD, programmed cell death SOD, superoxide dismutase |
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