油松成熟胚培养直接器官发生与植株再生

以油松（Pinus tabulaeformis Carr.）成熟合子胚为外植体进行了直接器官发生研究。在添加1~5 mg·L^-1 BA，或再配合添加1～10 mg·L^-1 2,4-D的DCR和MS培养基上均能诱导发生不定芽。在只添加1 mg·L^-1 BA的DCR培养基上不定芽诱导率最高，为90%。及时将不定芽继代于不含生长调节物质的DCR培养基上有利于芽的伸长。添加1 mg·L^-1 IBA的1/2DCR培养基最适于不定根的诱导，在该培养基上不定根诱导率达到47.1%。再生植株在蛭石基质上炼苗后转到温室培养，成活率高达86.4%，且生长正常。

Direct Organogenesis and Plantlet Regeneration from Mature Zygotic Embryos of Chinese Pine(Pinus tabulaeformis Carr.)

Mature zygotic embryos of Chinese pine were cultured as initial explants to investigate the process of direct organogenesis. Adventitious buds were initiated on DCR and MS medium supplemented with 1~5 mg·L^-1 benzyladenine (BA) with or without 1~10 mg·L^-1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest induction frequency of adventitious buds (90%) was obtained on DCR medium containing 1 mg·L^-1 BA. Subsequent transfer of buds to plant growth regulator (PGR)-free medium in time was advantageous for elongation of adventitious buds. 1/2DCR medium with 1 mg·L^-1 indole-3-butyric acid (IBA) was suitable for adventitious root initiation, on which average rooting frequency was 47.1%. Plantlets were successfully acclimatized in vermiculite and transferred to greenhouse, where they had a survival rate of 86.4% and no morphological abnormalities.