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Growth characteristics of Geotrichum ingens on propionic acid and acetic acid in batch and continuous culture
Authors:E. Vorster  S. G. Kilian  J. C. du Preez
Abstract:Growth of Geotrichum ingens in batch cultures was completely inhibited by 47 g acetic acid/l or 33 g propionic acid/I. With mixtures of acetic and propionic acids, however, growth only ceased at 55 g/l. Acetic acid inhibited growth linearly, whereas propionic acid inhibited growth non-linearly. In continuous culture, two steady states at each dilution rate were observed at high dilution rates for acetic acid and propionic acid. The highest yield coefficient (0.69 g cells/g substrate) was achieved with propionic acid as substrate. On both substrates and their mixtures, the protein content of the biomass increased when the dilution rate was increased.The authors are with the Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, Bloemfontein 9300, South Africa
Keywords:Acetic acid  chemostat  Geotrichum ingens  growth  inhibition  kinetics  monocarboxylic acids  propionic acid  yeast
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