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Use of Brassica callus culture to induce sporulation in Alternaria brassicae (Berk.) Sacc.
Authors:M. K. Joshi  U. S. Singh  G. K. Garg
Affiliation:(1) Dept. of Biochemistry, G.B. Pant University of Agriculture and Technology, 263 145 Pantnagar, UP, India;(2) Dept. of Plant Pathology, G.B. Pant University of Agriculture and Technology, 263 145 Pantnagar, UP, India
Abstract:A non-sporulating isolate of Alternaria brassicae, inoculated on callus culture of Brassica juncea cv. Kranti, colonized the callus and produced spores. When captafol, a fungicide, was added (100 mg/l) to the callus culture medium, if effectively checked fungal contamination and saprophytic growth of A. brassicae on culture medium, without adversely affecting callus growth or establishment of dual culture.
Keywords:Brassica juncea  Alternaria brassicae  captafol  dual culture  sporulation  callus
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