Use of Brassica callus culture to induce sporulation in Alternaria brassicae (Berk.) Sacc. |
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Authors: | M. K. Joshi U. S. Singh G. K. Garg |
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Affiliation: | (1) Dept. of Biochemistry, G.B. Pant University of Agriculture and Technology, 263 145 Pantnagar, UP, India;(2) Dept. of Plant Pathology, G.B. Pant University of Agriculture and Technology, 263 145 Pantnagar, UP, India |
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Abstract: | A non-sporulating isolate of Alternaria brassicae, inoculated on callus culture of Brassica juncea cv. Kranti, colonized the callus and produced spores. When captafol, a fungicide, was added (100 mg/l) to the callus culture medium, if effectively checked fungal contamination and saprophytic growth of A. brassicae on culture medium, without adversely affecting callus growth or establishment of dual culture. |
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Keywords: | Brassica juncea Alternaria brassicae captafol dual culture sporulation callus |
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