Exogenous ATP induces electrical membrane responses in fibroblasts |
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Authors: | Yasunobu Okada Toshihiko Yada Takako Ohno-Shosaku Shigetoshi Oiki Shunji Ueda Koichi Machida |
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Affiliation: | 1. Department of Physiology, Faculty of Medicine, Kyoto University, Kyoto 606, Japan;2. Department of Anesthesiology, Faculty of Medicine, Kyoto University, Kyoto 606, Japan;3. Department of Internal Medicine, Faculty of Medicine, Kyoto University, Kyoto 606, Japan;4. Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606, Japan |
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Abstract: | Mouse fibroblastic L cells responded to exogenous ATP (0.2 mM) with a transient hyperpolarization due to increased membrane permeability to K+. By contrast, intracellular injection of ATP (up to about 3 mM) produced no noticeable effects on the membrane potential. The effects of a non-hydrolysable analogue of ATP (AMP-PNP) were similar to those of ATP. After successive applications of ATP, the cell membrane became virtually unresponsive (desensitized). Extracellular ADP was also effective, but AMP or adenosine was not. Antazoline suppressed the ATP response. Thus, exogenous ATP and ADP appear to stimulate P2-purinoceptors. Similar responses to ATP (or ADP) were also observed in human normal diploid fibroblasts (Flow 1000 line). |
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Keywords: | To whom offprint requests should be sent. |
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