Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling |
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| Authors: | Rita L Strack Dibyendu Bhattacharyya Benjamin S Glick Robert J Keenan |
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| Affiliation: | (1) Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637, USA;(2) Department of Molecular Genetics and Cell Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, USA |
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| Abstract: | Background Whole-cell labeling is a common application of fluorescent proteins (FPs), but many red and orange FPs exhibit cytotoxicity that limits their use as whole-cell labels. Recently, a tetrameric red FP called DsRed-Express2 was engineered for enhanced solubility and was shown to be noncytotoxic in bacterial and mammalian cells. Our goal was to create derivatives of this protein with different spectral properties. |
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