SUBUNITS OF CHOLINE ACETYLTRANSFERASE (EC 2.3.1.6) |
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| Authors: | L-P Chao |
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| Institution: | Department of Neurology, Reed Neurological Research Center, UCLA School of Medicine, Los Angeles, CA 90024. U.S.A. |
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| Abstract: | Abstract— The molecular weight of choline acetyltransferase is determined to be approx 87,000-89,000 daltons. There are 808 amino acid residues per molecule of the enzyme with a calculated mol. wt of 89,300. Peptides of ChAc were studied after tryptic digestion, cyanogen bromide and BNPS-skatole cleavages. The results of these studies suggest that the enzyme contains 6 identical subunits with 133 amino acid residues each and with a calculated molecular weight of 14,700 daltons for the monomer. This conclusion is supported by the fact that serine is the only amino terminal residue and alanine is the only carboxyl-terminal group. |
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