Analysis of partial 16S rRNA nucleotide sequences of Chlamydia pecorum and C. psittaci |
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Authors: | Noreen Sheehya Bryan Markeya PJoseph Quinna |
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Institution: | University College Dublin, Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Ballsbridge, Dublin 4, Ireland |
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Abstract: | Partial 16S nucleotide sequences of Chlamydia psittaci isolates S26/3 (abortion), P94/1 (pigeon) and Chlamydia pecorum isolates W73 (enteric) and E58 (encephalomyelitis) were determined. Analysis of these data indicates very high levels of interspecies sequence conservation, with C. psittaci being more closely related to C. pecorum than to C. pneumoniae or C. trachomatis. Restriction enzyme analysis of nucleotide sequences indicated that BslI can be used to clearly distinguish C. psittaci and C. pecorum isolates. Psittacine and non-psittacine (pigeon) avian isolates of C. psittaci were distinguished using MaeI. |
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Keywords: | Chlamydia species Restriction enzyme Interspecies relationship |
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