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Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera
Authors:Magdalena Jacobson  Per Wallgren  Ann Nordengrahn  Malik Merza  Ulf Emanuelson
Affiliation:(1) Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Husbandry, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden;(2) National Veterinary Institute, 751 89 Uppsala, Sweden;(3) Svanova Biotech, 751 45 Uppsala, Sweden
Abstract:

Background  

Lawsonia intracellularis is a common cause of chronic diarrhoea and poor performance in young growing pigs. Diagnosis of this obligate intracellular bacterium is based on the demonstration of the microbe or microbial DNA in tissue specimens or faecal samples, or the demonstration of L. intracellularis-specific antibodies in sera. The aim of the present study was to evaluate a blocking ELISA in the detection of serum antibodies to L. intracellularis, by comparison to the previously widely used immunofluorescent antibody test (IFAT).
Keywords:
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