A next-generation sequencing method for overcoming the multiple gene copy problem in polyploid phylogenetics,applied to <Emphasis Type="Italic">Poa</Emphasis> grasses |
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Authors: | Philippa C Griffin Charles Robin Ary A Hoffmann |
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Institution: | (1) Department of Genetics, University of Melbourne, Parkville, 3010, Victoria, Australia;(2) Department of Zoology, University of Melbourne, Parkville, 3010, Victoria, Australia |
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Abstract: | Background Polyploidy is important from a phylogenetic perspective because of its immense past impact on evolution and its potential
future impact on diversification, survival and adaptation, especially in plants. Molecular population genetics studies of
polyploid organisms have been difficult because of problems in sequencing multiple-copy nuclear genes using Sanger sequencing.
This paper describes a method for sequencing a barcoded mixture of targeted gene regions using next-generation sequencing
methods to overcome these problems. |
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Keywords: | |
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