Chloroplast ultrastructure and thylakoid polypeptide composition are affected by different salt concentrations in the halophytic plant Arthrocnemum macrostachyum |
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Authors: | Trotta Andrea Redondo-Gómez Susana Pagliano Cristina Clemente Manuel Enrique Figueroa Rascio Nicoletta Rocca Nicoletta La Antonacci Alessia Andreucci Flora Barbato Roberto |
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Institution: | a Dipartimento di Scienze dell’Ambiente e della Vita, Università del Piemonte Orientale,, viale Teresa Michel 11, 15121 Alessandria, Italy b Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Apartado 1095, 41080 Sevilla, Spain c Dipartimento di Scienza dei Materiali e Ingegneria Chimica, Politecnico di Torino, Italy d Dipartimento di Biologia, Università di Padova, Padova, Italy |
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Abstract: | The effect of different external salt concentrations, from 0 mM to 1030 mM NaCl, on photosynthetic complexes and chloroplast ultrastructure in the halophyte Arthrocnemum macrostachyum was studied. Photosystem II, but not Photosystem I or cytochrome b6/f, was affected by salt treatment. We found that the PsbQ protein was never expressed, whereas the amounts of PsbP and PsbO were influenced by salt in a complex way. Analyses of Photosystem II intrinsic proteins showed an uneven degradation of subunits with a loss of about 50% of centres in the 0 mM NaCl treated sample. Also the shape of chloroplasts, as well as the organization of thylakoid membranes were affected by NaCl concentration, with many grana containing few thylakoids at 1030 mM NaCl and thicker grana and numerous swollen thylakoids at 0 mM NaCl. The PsbQ protein was found to be depleted also in thylakoids from other halophytes. |
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Keywords: | Chl chlorophyll PAM pulse amplitude modulated fluorimeter PS photosystem RC reaction centre SDS-PAGE sodium dodecyl sulphate polyacrylamide gel electrophoresis |
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