Functional reconstitution of an isolated sodium channel from bovine trachea |
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| Authors: | B D Cherksey |
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| Institution: | Department of Physiology and Biophysics, NYU School of Medicine, NY. |
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| Abstract: | An amiloride-sensitive Na+ channel from bovine trachea was isolated using an affinity gel and reconstituted into a planar lipid bilayer. This channel exhibited: 1. Fluctuations with long duration opening and closing times, weak voltage dependence, and a conductance of 6 pS. 2. Selectivity of at least 100-fold for Na+ over K+. 3. Saturates at a Na+ concentration of 90 mM. 4. Blocked by amiloride, 50% inhibition at 0.1 microM. |
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