| 角质细胞生长因子2对细胞生长、移行及创伤愈合的作用 |
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| 引用本文： | 陈霞,胡 炜,魏万贵,沈 雁,陈 燕,杨胜利,龚 毅. 角质细胞生长因子2对细胞生长、移行及创伤愈合的作用[J]. 生物化学与生物物理进展, 2009, 36(7): 854-862. DOI: 10.3724/SP.J.1206.2008.00724 |
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| 作者姓名： | 陈霞 胡 炜 魏万贵 沈 雁 陈 燕 杨胜利 龚 毅 |
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| 作者单位： | 1. 中国科学院上海生命科学研究院,上海,200233
2. 中国科学院研究生院,北京,100049 |
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| 基金项目： | 国家重点基础研究发展计划 |
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| 摘 要： | 角质细胞生长因子2(KGF-2)是成纤维细胞生长因子超家族的一员，由间质细胞合成并分泌，能特异促进上皮细胞增殖、分化与迁移，对脊椎动物多种组织和器官的发育起重要调控作用．通过PCR从人的肾组织cDNA文库中克隆分离获得了KGF-2的cDNA，表明该因子在成人肾中有表达．采用大肠杆菌表达并纯化重组蛋白用于生物学功能研究的结果显示：KGF-2在体外不仅能够促进角质细胞的生长和增殖，而且对其凋亡具有抑制作用，还对细胞的移行具有影响．在动物实验中，KGF-2能促进皮肤切除产生的伤口愈合，提示该蛋白质可以作为创伤治疗或辅助用药的候选分子．
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| 关 键 词： | 角质细胞生长因子2 细胞生长 细胞凋亡 细胞移行 伤口愈合 治疗应用 |
| 收稿时间： | 2008-10-22 |
| 修稿时间： | 2009-03-27 |
Effects of keratinocyte growth factor 2(KGF-2) on keratinocyte growth, migration and on excisional wound healing |
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| CHEN Xi,HU Wei,WEI Wan-Gui,SHEN Yan,CHEN Yan,YANG Sheng-Li and GONG Yi. Effects of keratinocyte growth factor 2(KGF-2) on keratinocyte growth, migration and on excisional wound healing[J]. Progress In Biochemistry and Biophysics, 2009, 36(7): 854-862. DOI: 10.3724/SP.J.1206.2008.00724 |
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| Authors: | CHEN Xi HU Wei WEI Wan-Gui SHEN Yan CHEN Yan YANG Sheng-Li GONG Yi |
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| Affiliation: | Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200233, China;Graduate University of The Chinese Academy of Sciences, Beijing 100049, China;Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200233, China;Graduate University of The Chinese Academy of Sciences, Beijing 100049, China;Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200233, China;Graduate University of The Chinese Academy of Sciences, Beijing 100049, China;Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200233, China;Graduate University of The Chinese Academy of Sciences, Beijing 100049, China;Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200233, China;Graduate University of The Chinese Academy of Sciences, Beijing 100049, China;Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200233, China;Graduate University of The Chinese Academy of Sciences, Beijing 100049, China;Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200233, China;Graduate University of The Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Keratinocyte growth factor 2 (KGF-2) is a member of the FGF family that is mainly synthesized by mesenchymal cells and acts predominantly on epithelial cells in a paracrine manner. It is known to play an important role in fetal limb and lung development; skin wound healing and prostatic epithelial cell growth. The KGF-2 coding sequence were isolated from human kidney cDNA library, revealing that the Kgf-2 gene is also expressed in the kidney apparatus. Purified from prokaryotic E. coli cells, the effects of the recombinant KGF-2 protein in cultured keratinocyte were analyzed by using MTT assay and in situ TUNEL assay. Interestingly, results revealed that KGF-2 promoted keratinocyte cell growth by stimulating cell proliferation and attenuating cell apoptosis. These findings supported a few evidences that KGF-2 could contribute to alveolar epithelial cells against apoptosis. Cell migration assays for the first time revealed that KGF-2 could stimulate keratinocyte cell migration in vitro. In addition, in the pilot animal test, recombinant KGF-2 incorporated within the hydrogel dressing exhibited significantly stimulatory effect on cutaneous wound healing. These combined effects implicate a potential therapeutic application of human recombinant KGF-2 in the future. |
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| Keywords: | KGF-2 keratinocyte cell proliferation cell apoptosis cell migration wound healing therapeutic application |
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