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Eight novel microsatellite markers from the Père David’s deer (<Emphasis Type="Italic">Elaphurus davidianus</Emphasis>)
Authors:Yang Jiao  Yun-Fa Ge  Sheng-Guo Fang
Institution:(1) College of Life Sciences, Zhejiang University, Hangzhou, 310058, P.R. China;(2) State Conservation Center for Gene Resources of Endangered Wildlife and the Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Hangzhou, 310058, P.R. China
Abstract:Père David’s deer underwent known bottlenecks but has recovered to more than 2000 individuals in China, making it interesting to assess its genetic variability from a microsatellite perspective. For this purpose, we developed eight novel microsatellite loci using magnetic-bead enrichment methods. These microsatellite markers revealed a low level of genetic variation in the David’s deer, showing two alleles each locus, 0.31 and 0.38 for average observed and expected heterozyogosities, respectively. Combined with previous cross-species primers, this set of markers would play an important role in future genetic investigation of the David’s deer.
Keywords:Père David’  s deer  Microsatellite  Magnetic bead  Allele  Heterozygosity
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